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Postdoctoral Research Associate, Proteomics, Molecular Biology, and Immunology
Southern California Food Allergy Institute in the Long Beach, California
 
 
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Date Posted 06/29/2020
Category
Postdoctoral-Biology
Employment Type Full-time
Application Deadline Open until filled
 
 
 
 
 

Job Description
TPIRC is a rare disease institute equipped with a state-of-the art research facility where discovery is driven by the study of millions upon millions of data points. With each case that we treat, we continue to grow our data sets at an exponential rate. This form of data collection and analysis opens the door for truly patient-driven research derived from real-world, clinical data. This collection of shared information offers the greatest hope to truly advance treatment protocols for some of the most complex conditions.

A post-doctoral research associate is sought to work on the purification and immunochemical and biochemical characterization of food allergens. The qualified applicant will manage a project dealing with allergen purification and reactivity and specifically include the seed storage proteins, the major allergenic proteins in plant seed, and their post-translational modification (including phosphorylation and glycosylation).

Experienced in project management, general molecular biological and proteomic techniques, in particular liquid chromatography, mass spectrometry, ELISA, immunoblotting, post- translational modification elucidation (kinase assays and glycan analysis), recombinant protein expression and biophysical and biochemical protein characterization.

Responsibilities

  • Characterize the proteomes of various allergenic food sources

  • Extract protein via various methods

  • Work with multidisciplinary teams to support the patient driven research in several

  • different disease models.

  • Maintain experimental laboratory notebook and help with ordering/cataloging of

  • reagents and supplies.

  • Optimize immune cell isolation, characterization, activation, transduction and

  • expansion.

  • Requirements

  • PhD degree in biological sciences, proteomics, botany or related field.

  • Purification and immunochemical and biochemical characterization of food allergens.

  • Experience with Western blot analysis, column chromatography, mass spectrometry,

  • immunological assays (ELISA), and multicolor flow cytometry analysis.

  • Experience with mammalian cell culture, cell transfection, transduction and stable cell

  • line generation.

  • Experience with molecular biology techniques such as DNA preparation, PCR/qPCR

  • and molecular cloning.

  • Excellent interpersonal, verbal and written communication skills in this collaborative work environment.

  • Comfortable in a fast-paced small company environment with minimal supervision.

Competitive salary and excellent work environment are offered. Self-motivated individuals with a PhD in biological sciences or related field. Especially those who are skilled in immunology, bioinformatics, or pulmonology are encouraged to apply. Must include a cover letter and CV when you apply.

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